Detection of protozoal Trichomonas vaginalis and abnormal vaginal flora in high vaginal smear
نویسندگان
چکیده
منابع مشابه
Simultaneous isolation of Trichomonas vaginalis and collection of vaginal exudate.
For the purpose of immunological study of Trichomonas vaginalis infestation in man, methods were required for the simultaneous isolation of organisms and for the collection of material which might contain antibodies against the organisms. The present paper describes the method developed for females and relates it to the usual routine method for diagnosis of this infection, by microscopical exam...
متن کاملUse of an immunochromatographic assay for rapid detection of Trichomonas vaginalis in vaginal specimens.
Trichomonas vaginalis infection is estimated to be the most widely prevalent nonviral sexually transmitted infection in the world. Wet-mount microscopy is the most common diagnostic method, although it is less sensitive than culture. The OSOM Trichomonas Rapid Test (Genzyme Diagnostics, Cambridge, Mass.) (referred to here as OSOM) is a new point-of-care diagnostic assay for T. vaginalis that us...
متن کاملEvaluation of acridine orange stain for detection of Trichomonas vaginalis in vaginal specimens.
Vaginal exudates from 105 patients were examined by direct wet-mount microscopy and acridine orange stain for the presence of Trichomonas vaginalis. Both procedures demonstrated greater than 90% agreement in both sensitivity and specificity. When specimens can be examined immediately after collection, it appears that wet-mount microscopy is almost as sensitive as acridine orange staining for de...
متن کاملColorimetric one-tube nested PCR for detection of Trichomonas vaginalis in vaginal discharge.
A colorimetric one-tube nested PCR was developed for the detection of Trichomonas vaginalis in clinical vaginal discharge specimens. A family of 650-bp specific DNA repeats from the T. vaginalis genome was targeted. There was no cross-reaction with human DNA or other infectious agents, including Pentatrichomonas hominis and Giardia lamblia. The colorimetric assay was applied as an adjunct to ne...
متن کاملDetection of Trichomonas vaginalis in vaginal specimens by direct immunofluorescence assay.
Vaginal discharge specimens from 105 women were examined by wet mount, culture, and a new direct immunofluorescence assay to detect Trichomonas vaginalis. The organism was detected by culture in 31 patients, by direct immunofluorescence in 26 patients, and by wet mount in 21 patients.
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ژورنال
عنوان ژورنال: International Journal of Reproduction, Contraception, Obstetrics and Gynecology
سال: 2017
ISSN: 2320-1789,2320-1770
DOI: 10.18203/2320-1770.ijrcog20170382